CpG Methylation Modifies the Genetic Stability of Cloned Repeat Sequences
نویسندگان
چکیده
منابع مشابه
CpG methylation modifies the genetic stability of cloned repeat sequences.
The genetic stability of tandemly repeated DNAs is affected by repeat sequence, tract length, tract purity, and replication direction. Alterations in DNA methylation status are thought to influence many processes of mutagenesis. By use of bacterial and primate cell systems, we have determined the effect of CpG methylation on the genetic stability of cloned di-, tri-, penta- and minisatellite re...
متن کاملPredicting DNA Methylation Susceptibility Using CpG Flanking Sequences
DNA methylation is a type of chemical modification of DNA that adds a methyl group to DNA at the fifth carbon of the cytosine pyrimidine ring. In normal cells, methylation of CpG dinucleotides is extensively found across the genome. However, specific DNA regions known as the CpG islands, short CpG dinucleotide-rich stretches (500 bp - 2000bp), are commonly unmethylated. During tumorigenesis, on...
متن کاملMETHYLATION OF ThE ANDROGEN RECEPTOR CPG ISLAND
Survivinis a memberofthe inhibitor ofapoptosis protein (IAP)family. WeInvestigated the antiapoptotic mechanism ofSurvivin, as well as its expressionIn 60 human tumor cell lines used for the National Cancer lontitute's anticancer drag screening program, In cotransfection experhnents, cell deathInduced by Bax or Faa (CD 95) was partially inhibited (mean ±SD,65%±8%)bySurv...
متن کاملExpanded CTG repeat demarcates a boundary for abnormal CpG methylation in myotonic dystrophy patient tissues.
Myotonic dystrophy (DM1) affects multiple organs, shows age-dependent progression and is caused by CTG expansions at the DM1 locus. We determined the DM1 CpG methylation profile and CTG length in tissues from DM1 foetuses, DM1 adults, non-affected individuals and transgenic DM1 mice. Analysis included CTCF binding sites upstream and downstream of the CTG tract, as methylation-sensitive CTCF bin...
متن کاملCpG methylation reduces genomic instability.
Hypomethylation of DNA in tumor cells is associated with genomic instability and has been suggested to be due to activation of mitotic recombination. We have studied the methylation patterns in two 650 kb double minute chromosomes present in two mouse tumor cell lines, resistant to methotrexate. Multiple copies of the double minute chromosomes amplifying the dihydrofolate reductase gene are pre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Research
سال: 2002
ISSN: 1088-9051
DOI: 10.1101/gr.74502